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Children's Hospital, University of Mainz, Germany.
We have determined the nucleotide sequence of the human fibroblast growth factor receptor 3 (FGFR3) gene, including 800 bp of the 5'-flanking region and compared the sequence with the previously published murine Fgfr3 gene. The organization of the gene is highly conserved between man and mouse. We used the intron sequences to design a set of primers that allow amplification of the 17 exons (2-18) that encode the complete open reading frame. Using these primers the FGFR3 gene can be amplified at the genomic level, which significantly facilitates mutational screening.
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