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Cloning and characterization of the 5' flanking sequences (promoter region) of the human GLP-1 receptor gene.
Department of Internal Medicine, Philipps-University of Marburg, Germany.
The glucagon-like peptide 1 (7-36) amide (GLP-1) receptor mediates the insulinotropic effects of the incretin hormone GLP-1. To elucidate the tissue-specific regulation of the GLP-1 receptor we screened a human genomic library with a human GLP-1 receptor cDNA. The gene spans 40 kb and consists of at least seven exons. The promoter contained no TATA- or CAAT-boxes, but several other putative cis-regulatory recognition sequences including three Sp1 binding sites. Transient transfections of GLP-1 receptor producing and non-producing cells with promoter/ reporter gene constructs revealed that the putative Sp1 binding sites and several other silencer and tissue specific elements are important for the activity. Therefore, 3000 bp upstream the GLP-1 receptor coding sequences comprise regulatory elements essential for the tissue- and cell-specific transcription of the gene.
PMID: 9213353 [PubMed - indexed for MEDLINE]
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Cited by 3 PubMed Central articles
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Variants in the FFAR1 gene are associated with beta cell function.
Kalis M, Levéen P, Lyssenko V, Almgren P, Groop L, Cilio CM.
PLoS One. 2007 Nov 7; 2(11):e1090. Epub 2007 Nov 7.
[PLoS One. 2007]
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Characterisation of the mouse vasoactive intestinal peptide receptor type 2 gene, Vipr2, and identification of a polymorphic LINE-1-like sequence that confers altered promoter activity.
Steel G, Lutz EM.
J Neuroendocrinol. 2007 Jan; 19(1):14-25.
[J Neuroendocrinol. 2007]
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The human vasoactive intestinal peptide/pituitary adenylate cyclase-activating peptide receptor 1 (VPAC1) promoter: characterization and role in receptor expression during enterocytic differentiation of the colon cancer cell line Caco-2Cl.20.
Couvineau A, Maoret JJ, Rouyer-Fessard C, Carrero I, Laburthe M.
Biochem J. 2000 May 1; 347 Pt 3:623-32.
[Biochem J. 2000]