Abstract
In an attempt to identify new nuclear genes involved in the synthesis and processing of mitochondrial tRNAs, we utilized a multicopy nuclear library to suppress the heat-sensitive phenotype of a Saccharomyces cerevisiae mitochondrial mutant strain. This strain (Ts 932) is defective in the 3'-end processing of the mitochondrial tRNAAsp transcript. The nuclear genes coding for the mitochondrial elongation factor Tuf M and for the mitochondrial aspartyl-tRNA synthetase have been found to restore the temperature-resistant phenotype and to correct the RNA processing defect. Suppression was effective even when the genes were present on a centromeric plasmid.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aspartate-tRNA Ligase / genetics*
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Blotting, Northern
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Cell Division / genetics
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DNA Transposable Elements
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DNA, Mitochondrial / genetics*
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Fungal Proteins / genetics
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Gene Dosage*
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Gene Expression Regulation, Fungal
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Mutagenesis
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Mutation
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Oxidoreductases
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Peptide Elongation Factor Tu / genetics*
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Phenotype
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Plasmids / genetics
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RNA Processing, Post-Transcriptional
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RNA, Transfer, Asp / genetics*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / growth & development
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Saccharomyces cerevisiae / metabolism
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Saccharomyces cerevisiae Proteins*
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Temperature
Substances
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DNA Transposable Elements
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DNA, Mitochondrial
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Fungal Proteins
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RNA, Transfer, Asp
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Saccharomyces cerevisiae Proteins
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Oxidoreductases
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SMM1 protein, S cerevisiae
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Peptide Elongation Factor Tu
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Aspartate-tRNA Ligase