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Life Sci. 1997;60(24):2221-30.

Hydrogen peroxide-mediated cytotoxicity to cultured colonic epithelial cells.

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  • 1Department of Gastroenterology, Department of Veterans Affairs Medical Center, Long Beach, California 90822, USA.

Abstract

Reactive oxygen metabolites (ROM) contribute to colonic cellular injury, in certain pathophysiological conditions. We investigated the role of iron and individual metabolites in their cytotoxicity to cultured colonic epithelial cells from adult white rabbits. Reactive oxygen metabolites, enzymatically generated by hypoxanthine/xanthine oxidase, have a direct cytotoxic effect on cultured colonic epithelial cells. This cellular injury was inhibited by catalase but not SOD. Damage was not aggravated by ferrous iron or EDTA-chelated iron. Such damage was prevented by chelating intracellular iron, but not extracellular iron. These results suggest that H2O2 is more toxic to colonic epithelial cells than 02.- and OH. in the extracellular space. H2O2 enter the intracellular space and is converted to the more reactive and harmful OH. leading to cellular injury in the presence of intracellular iron.

PMID:
9188765
[PubMed - indexed for MEDLINE]
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