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    Science. 1997 Jun 20;276(5320):1878-81.

    Activation of the G protein Gq/11 through tyrosine phosphorylation of the alpha subunit.

    Source

    Department of Oncology, Institute of Medical Science, University of Tokyo, Tokyo 108, Japan.

    Abstract

    Various receptors coupled to the heterotrimeric guanine nucleotide-binding protein Gq/11 stimulate formation of inositol-1,4,5-trisphosphate (IP3). Activation of these receptors also induces protein tyrosine phosphorylation. Formation of IP3 in response to stimulated receptors that couple to Gq/11 was blocked by protein tyrosine kinase inhibitors. These inhibitors appeared to act before activation of Gq/11. Moreover, stimulation of receptors coupled to Gq/11 induced phosphorylation on a tyrosine residue (Tyr356) of the Galphaq/11 subunit, and this tyrosine phosphorylation event was essential for Gq/11 activation. Tyrosine phosphorylation of Galphaq/11 induced changes in its interaction with receptors. Therefore, tyrosine phosphorylation of Galphaq/11 appears to regulate the activation of Gq/11 protein.

    PMID:
    9188537
    [PubMed - indexed for MEDLINE]

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