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Cell. 1997 May 30;89(5):799-809.

Site-specific pseudouridine formation in preribosomal RNA is guided by small nucleolar RNAs.

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  • 1Laboratoire de Biologie Mol√©culaire du CNRS, Universit√© Paul Sabatier, Toulouse, France.

Abstract

During the nucleolar maturation of eukaryotic ribosomal RNAs, many selected uridines are converted into pseudouridine by a thus far undefined mechanism. The nucleolus contains a large number of small RNAs (snoRNAs) that share two conserved sequence elements, box H and ACA. In this study, we demonstrate that site-specific pseudouridylation of rRNAs relies on short ribosomal signal sequences that are complementary to sequences in box H/ACA snoRNAs. Genetic depletion and reconstitution studies on yeast snR5 and snR36 snoRNAs demonstrate that box H/ACA snoRNAs function as guide RNAs in rRNA pseudouridylation. These results define a novel function for snoRNAs and further reinforce the idea that base pairing is the most common way to obtain specific substrate-"enzyme" interactions during rRNA maturation.

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PMID:
9182768
[PubMed - indexed for MEDLINE]
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