High level expression of p27(kip1) and cyclin D1 in some human breast cancer cells: inverse correlation between the expression of p27(kip1) and degree of malignancy in human breast and colorectal cancers

S Fredersdorf; J Burns; A M Milne; G Packham; L Fallis; C E Gillett; J A Royds; D Peston; P A Hall; A M Hanby; D M Barnes; S Shousha; M J O'Hare; X Lu

doi:10.1073/pnas.94.12.6380

High level expression of p27(kip1) and cyclin D1 in some human breast cancer cells: inverse correlation between the expression of p27(kip1) and degree of malignancy in human breast and colorectal cancers

Proc Natl Acad Sci U S A. 1997 Jun 10;94(12):6380-5. doi: 10.1073/pnas.94.12.6380.

Authors

S Fredersdorf¹, J Burns, A M Milne, G Packham, L Fallis, C E Gillett, J A Royds, D Peston, P A Hall, A M Hanby, D M Barnes, S Shousha, M J O'Hare, X Lu

Affiliation

¹ Ludwig Institute for Cancer Research, Imperial College School of Medicine at St. Mary's, Norfolk Place, London, W2 1PG, United Kingdom.

Abstract

The expression of cyclin-dependent kinase inhibitor p27(kip1) in human tumors and normal tissues was investigated using a panel of novel anti-p27(kip1) mAbs. An inverse correlation between expression of p27(kip1) and cell proliferation was generally observed after analyzing its expression in 25 different normal human tissues. In some highly proliferative human breast cancer cells, however, high level p27(kip1) expression was seen, indicating the existence of a mechanism by which some growing tumor cells may tolerate this inhibitor of cell cycle progression. Detailed studies demonstrated a correlation between the high level expression of p27(kip1) and cyclin D1 in human breast cancer cells. There was also an inverse correlation between the expression of p27(kip1) and the degree of tumor malignancy in human breast and colorectal cancers, indicating that p27(kip1) may be a useful prognostic marker in these cancers.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomarkers, Tumor
Breast Neoplasms / metabolism*
Breast Neoplasms / pathology*
Cell Cycle
Cell Cycle Proteins*
Cell Division
Colorectal Neoplasms / metabolism*
Colorectal Neoplasms / pathology*
Cyclin D1
Cyclin-Dependent Kinase Inhibitor p27
Cyclin-Dependent Kinases / antagonists & inhibitors
Cyclins / analysis
Cyclins / biosynthesis*
Female
Genes, Tumor Suppressor
Humans
Immunohistochemistry
Kinetics
Male
Microtubule-Associated Proteins / analysis
Microtubule-Associated Proteins / biosynthesis*
Oncogene Proteins / analysis
Oncogene Proteins / biosynthesis*
Organ Specificity
Prognosis
Reference Values
Tumor Cells, Cultured
Tumor Suppressor Proteins*

Substances

Biomarkers, Tumor
Cell Cycle Proteins
Cyclins
Microtubule-Associated Proteins
Oncogene Proteins
Tumor Suppressor Proteins
Cyclin D1
Cyclin-Dependent Kinase Inhibitor p27
Cyclin-Dependent Kinases