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Appl Environ Microbiol. 1997 Jun;63(6):2460-3.

Detection of hepatitis A virus RNA in oyster meat.

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  • 1Hepatitis Branch (World Health Organization Collaborating Center), National Centers for Disease Control and Prevention, Atlanta, Georgia, USA.


Detection of low concentrations of viruses in shellfish is possible with nucleic acid amplification by PCR. Hepatitis A virus (HAV) has been detected in oyster meat by reverse transcription-PCR (RT-PCR). We developed a method to identify HAV RNA by RT-PCR of total RNA extracted from oyster meat contaminated by adsorption, bioaccumulation, or injection. With dot blot hybridization detection of amplicons from the RT-PCR, rapid screening of a large number of samples is feasible. As few as 8 PFU of HAV/g of oyster meat can be detected.

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