The central line representing S. cerevisiae chromosome V is marked in kilobase pairs, starts at the left at the guanine of the Sau3A site of the leftmost recombinant yeast DNA, and extends to the right to 570 kb. The centromere, CEN, is represented by a solid circle at ~151 kb. Above the line, placed across their map positions, are the individual recombinant yeast DNAs that were sequenced; 16 cosmids (thin lines), 8 lambdas (thick lines), and 1 plasmid (very thick line). Genbank accession numbers are placed below the line, above the bars indicating the corresponding map positions. From left to right, Genbank accession numbers are U18795, U18779, U18530, U18778, U18796, U18813, U18814, U18839, U18916, U18917 and U18922. There is some deliberate overlap between Genbank entries to maintain contiguity.

The bottom line is a schematic map of yeast chromosome V from 240 kb to 310 kb. Individual, sequenced recombinant DNAs are placed above the line and across the appropriate map positions. Cosmid 9380 covers map positions ~240 kb to 300 kb and overlaps lambda 3612. The latter is the only recombinant DNA in the entire Olson collection4,14 that covers map positions 300 kb to 310 kb. Because 9380 could only be produced in minute amounts, three lambda DNAs (thick lines) were substituted: 4678, 6592 and 4742. The X marks the position of the ~2 kb deletion in 6592 DNA.