Nucleic Acids Res. 1997 Jun 1;25(11):2062-7.

Mapping of Stat3 serine phosphorylation to a single residue (727) and evidence that serine phosphorylation has no influence on DNA binding of Stat1 and Stat3.

Source

Laboratory of Molecular Cell Biology, The Rockefeller University, New York, NY 10021, USA.

Abstract

During their polypeptide ligand-induced activation Stats (signaltransducers andactivators oftranscription) 1 and 3 acquire, in addition to an obligatory tyrosine phosphorylation, phosphorylation on serine which boosts their transactivating potential [Wen, Z., Zhong, Z. and Darnell, J. E. Jr. (1995) Cell 82, 241-250]. By examining phosphopeptide maps of wild-type and mutant protein we show here that the Stat3 serine phosphorylation, like the Stat1 serine phosphorylation, occurs on a single residue, serine 727. Neither the DNA binding of Stat1 nor Stat3 is demonstrably affected by the presence or absence of the serine phosphorylation. Thus the earlier demonstration that transcription is enhanced by the presence of the serine 727 residue likely occurs after DNA binding. These findings do not agree with earlier claims of excess serine to tyrosine phosphorylation in activated Stats 1 and 3 or to claims of more stable DNA binding of serine phosphorylated Stat dimers.

PMID:: 9153303; [PubMed - indexed for MEDLINE]
PMCID: PMC146718

Free PMC Article

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Molecular Biology Databases

Research Materials

Get Article's Plasmids - Addgene

Supplemental Content

Related citations

Requirement of serine phosphorylation for formation of STAT-promoter complexes. [Science. 1995]
Requirement of serine phosphorylation for formation of STAT-promoter complexes.
Zhang X, Blenis J, Li HC, Schindler C, Chen-Kiang S. Science. 1995 Mar 31; 267(5206):1990-4.
Interleukin-13 induction of 15-lipoxygenase gene expression requires p38 mitogen-activated protein kinase-mediated serine 727 phosphorylation of Stat1 and Stat3. [Mol Cell Biol. 2003]
Interleukin-13 induction of 15-lipoxygenase gene expression requires p38 mitogen-activated protein kinase-mediated serine 727 phosphorylation of Stat1 and Stat3.
Xu B, Bhattacharjee A, Roy B, Xu HM, Anthony D, Frank DA, Feldman GM, Cathcart MK. Mol Cell Biol. 2003 Jun; 23(11):3918-28.
STAT3 serine phosphorylation by ERK-dependent and -independent pathways negatively modulates its tyrosine phosphorylation. [Mol Cell Biol. 1997]
STAT3 serine phosphorylation by ERK-dependent and -independent pathways negatively modulates its tyrosine phosphorylation.
Chung J, Uchida E, Grammer TC, Blenis J. Mol Cell Biol. 1997 Nov; 17(11):6508-16.
Review The role of STATs in transcriptional control and their impact on cellular function. [Oncogene. 2000]
Review The role of STATs in transcriptional control and their impact on cellular function.
Bromberg J, Darnell JE Jr. Oncogene. 2000 May 15; 19(21):2468-73.
Review STAT signaling in the pathogenesis and treatment of cancer. [Mol Med. 1999]
Review STAT signaling in the pathogenesis and treatment of cancer.
Frank DA. Mol Med. 1999 Jul; 5(7):432-56.

See reviews... See all...

Cited by 41 PubMed Central articles

Heteroarylketones inhibit astroglial interleukin-6 expression via a STAT3/NF-κB signaling pathway. [J Neuroinflammation. 2011]
Heteroarylketones inhibit astroglial interleukin-6 expression via a STAT3/NF-κB signaling pathway.
Schulz I, Engel C, Niestroj AJ, Zeitschel U, Menge K, Kehlen A, Meyer A, Rossner S, Demuth HU. J Neuroinflammation. 2011 Jul 29; 8:86. Epub 2011 Jul 29.
Two naturally occurring terpenes, dehydrocostuslactone and costunolide, decrease intracellular GSH content and inhibit STAT3 activation. [PLoS One. 2011]
Two naturally occurring terpenes, dehydrocostuslactone and costunolide, decrease intracellular GSH content and inhibit STAT3 activation.
Butturini E, Cavalieri E, de Prati AC, Darra E, Rigo A, Shoji K, Murayama N, Yamazaki H, Watanabe Y, Suzuki H, et al. PLoS One. 2011; 6(5):e20174. Epub 2011 May 18.
Polycystin-1 regulates STAT activity by a dual mechanism. [Proc Natl Acad Sci U S A. 2011]
Polycystin-1 regulates STAT activity by a dual mechanism.
Talbot JJ, Shillingford JM, Vasanth S, Doerr N, Mukherjee S, Kinter MT, Watnick T, Weimbs T. Proc Natl Acad Sci U S A. 2011 May 10; 108(19):7985-90. Epub 2011 Apr 25.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Mapping of Stat3 serine phosphorylation to a single residue (727) and evidence t...
Mapping of Stat3 serine phosphorylation to a single residue (727) and evidence that serine phosphorylation has no influence on DNA binding of Stat1 and Stat3.
Nucleic Acids Res. 1997 Jun 1 ;25(11):2062-7.

PubMed
Absence of evidence is not evidence of absence.
Absence of evidence is not evidence of absence.
BMJ. 1995 Aug 19 ;311(7003):485.

PubMed
Regular consumption of dark chocolate is associated with low serum concentration...
Regular consumption of dark chocolate is associated with low serum concentrations of C-reactive protein in a healthy Italian population.
J Nutr. 2008 Oct ;138(10):1939-45.

PubMed
The fidelity of DNA synthesis by eukaryotic replicative and translesion synthesi...
The fidelity of DNA synthesis by eukaryotic replicative and translesion synthesis polymerases.
Cell Res. 2008 Jan ;18(1):148-61.

PubMed
Effect of screening and adjuvant therapy on mortality from breast cancer.
Effect of screening and adjuvant therapy on mortality from breast cancer.
N Engl J Med. 2005 Oct 27 ;353(17):1784-92.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Display Settings:

Send to:

Mapping of Stat3 serine phosphorylation to a single residue (727) and evidence that serine phosphorylation has no influence on DNA binding of Stat1 and Stat3.

Source

Abstract

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Grant Support

LinkOut - more resources

Full Text Sources

Other Literature Sources

Molecular Biology Databases

Research Materials

Supplemental Content

Related citations

Cited by 41 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information