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Cell. 1997 May 2;89(3):445-55.

The vesicle docking protein p115 binds GM130, a cis-Golgi matrix protein, in a mitotically regulated manner.

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  • 1Cell Biology Laboratory, Imperial Cancer Research Fund, London, United Kingdom.

Abstract

The docking of transport vesicles with their target membrane is thought to be mediated by p115. We show here that GM130, a cis-Golgi matrix protein, interacts specifically with p115 and so could provide a membrane docking site. Deletion analysis showed that the N-terminus binds to p115, whereas the C-terminus binds to Golgi membranes. Mitotic phosphorylation of GM130 or a peptide derived from the N-terminus prevented binding to p115. The peptide also inhibited the NSF- but not the p97-dependent reassembly of Golgi cisternae from mitotic fragments, unless it was mitotically phosphorylated. Together, these data provide a molecular explanation for the COPI-mediated fragmentation of the Golgi apparatus at the onset of mitosis.

PMID:
9150144
[PubMed - indexed for MEDLINE]
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