Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Int J Obes Relat Metab Disord. 1997 Apr;21(4):261-6.

ASP stimulates glucose transport in cultured human adipocytes.

Author information

  • 1McGill Unit for the Prevention of Cardiovascular Disease, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada.

Abstract

OBJECTIVE:

The purpose of the present study was to examine the effect of Acylation Stimulating Protein (ASP) on glucose transport in cultured subcutaneous adipocytes.

DESIGN AND SUBJECTS:

Subcutaneous adipose tissue was obtained from non-obese, healthy females (18-32 y old) undergoing mammoplasty reduction. Preadipocytes were isolated and differentiated into adipocytes.

MEASUREMENTS:

Following the exposure of preadipocytes and adipocytes to ASP or insulin, glucose transport was assessed as [3H] 2-deoxy glucose uptake. The measurements were normalised per total cell protein.

RESULTS:

ASP increases specific membrane glucose transport in both preadipocytes and adipocytes in a time and concentration dependent manner. Stimulation in both cell types is rapid (within minutes), reaching a maximal effect between 1 and 4 h. However, after 24 h exposure to ASP, there is a downregulation in the response. The ASP response is greater following differentiation of preadipocytes to adipocytes and is compared to that of insulin. Dose response studies demonstrated a five-fold greater sensitivity of adipocytes (half-maximal concentration of ASP on adipocytes = 0.5 microM, preadipocytes = 2.3 microM).

CONCLUSION:

These results demonstrate that ASP not only stimulates triglyceride synthesis, but also glucose transport in differentiated human adipocytes and is consistent with a physiologically important role for ASP in postprandial energy storage.

PMID:
9130021
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Write to the Help Desk