Nucleic Acids Res. 1997 May 15;25(10):2037-8.

Enhanced RACE method using specific enrichment by biotinylated oligonucleotides bound to streptavidin coated magnetic particles.

Source

Lehrstuhl für Zellphysiologie, Ruhr-Universität Bochum, Universitätsstrasse 150, D-44780 Bochum, Germany.

Abstract

RACE (rapid amplification of cDNA ends) is commonly used for identification and isolation of 3'and 5'termini of cDNA. We developed an improvement of the RACE-method that allows the enrichment of wanted fragments. The important new feature is the purification of the amplified products by biotinylated oligonucleotides that hybridize internally. Hybrids are isolated by streptavidin coated magnetic particles.

PMID:: 9115376; [PubMed - indexed for MEDLINE]
PMCID:: PMC146681

Free PMC Article

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

BIOTIN - HSDB

Supplemental Content

Save items

Related citations in PubMed

Multiple isoform recovery (MIR)-PCR: a simple method for the isolation of related mRNA isoforms. [Nucleic Acids Res. 1998]
Multiple isoform recovery (MIR)-PCR: a simple method for the isolation of related mRNA isoforms.
Fagotti A, Gabbiani G, Pascolini R, Neuville P. Nucleic Acids Res. 1998 Apr 15; 26(8):2031-3.
Subtraction hybridization cDNA libraries. [Methods Mol Biol. 1995]
Subtraction hybridization cDNA libraries.
Schweinfest CW, Nelson PS, Graber MW, Demopoulos RI, Papas TS. Methods Mol Biol. 1995; 37:13-30.
Enrichment of microsatellites from the citrus genome using biotinylated oligonucleotide sequences bound to streptavidin-coated magnetic particles. [Biotechniques. 1994]
Enrichment of microsatellites from the citrus genome using biotinylated oligonucleotide sequences bound to streptavidin-coated magnetic particles.
Kijas JM, Fowler JC, Garbett CA, Thomas MR. Biotechniques. 1994 Apr; 16(4):656-60, 662.
Review [Rapid amplification of cDNA end and its application]. [Yi Chuan. 2004]
Review [Rapid amplification of cDNA end and its application].
Wang SL, Sheng CF, Qiao CL. Yi Chuan. 2004 May; 26(3):419-23.
Review [Isolation of mRNA 5'-end using oigo-capping]. [Tanpakushitsu Kakusan Koso. 1996]
Review [Isolation of mRNA 5'-end using oigo-capping].
Suzuki Y, Sugano S. Tanpakushitsu Kakusan Koso. 1996 Apr; 41(5):603-7.

See reviews... See all...

Cited by 2 PubMed Central articles

Sequence and expression characteristics of a nuclear-encoded chloroplast sigma factor from mustard (Sinapis alba). [Nucleic Acids Res. 1998]
Sequence and expression characteristics of a nuclear-encoded chloroplast sigma factor from mustard (Sinapis alba).
Kestermann M, Neukirchen S, Kloppstech K, Link G. Nucleic Acids Res. 1998 Jun 1; 26(11):2747-53.
Multiple isoform recovery (MIR)-PCR: a simple method for the isolation of related mRNA isoforms. [Nucleic Acids Res. 1998]
Multiple isoform recovery (MIR)-PCR: a simple method for the isolation of related mRNA isoforms.
Fagotti A, Gabbiani G, Pascolini R, Neuville P. Nucleic Acids Res. 1998 Apr 15; 26(8):2031-3.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Enhanced RACE method using specific enrichment by biotinylated oligonucleotides ...
Enhanced RACE method using specific enrichment by biotinylated oligonucleotides bound to streptavidin coated magnetic particles.
Nucleic Acids Res. 1997 May 15 ;25(10):2037-8.

PubMed
Identification of a protein kinase from Dictyostelium with homology to the novel...
Identification of a protein kinase from Dictyostelium with homology to the novel catalytic domain of myosin heavy chain kinase A.
J Biol Chem. 1997 May 2 ;272(18):11812-5.

PubMed
Molecular evolutionary analysis of the thiamine-diphosphate-dependent enzyme, tr...
Molecular evolutionary analysis of the thiamine-diphosphate-dependent enzyme, transketolase.
J Mol Evol. 1997 May ;44(5):552-72.

PubMed
Serum dioxin and diabetes mellitus in veterans of Operation Ranch Hand.
Serum dioxin and diabetes mellitus in veterans of Operation Ranch Hand.
Epidemiology. 1997 May ;8(3):252-8.

PubMed
Liver transplantation for metastatic neuroendocrine tumors.
Liver transplantation for metastatic neuroendocrine tumors.
Ann Surg. 1997 Apr ;225(4):347-54.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Enhanced RACE method using specific enrichment by biotinylated oligonucleotides bound to streptavidin coated magnetic particles.

Source

Abstract

MeSH Terms, Substances

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

Supplemental Content

Save items

Related citations in PubMed

Cited by 2 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information