Cell. 1997 Apr 4;89(1):105-14.

The G beta gamma sensitivity of a PI3K is dependent upon a tightly associated adaptor, p101.

Stephens LR, Eguinoa A, Erdjument-Bromage H, Lui M, Cooke F, Coadwell J, Smrcka AS, Thelen M, Cadwallader K, Tempst P, Hawkins PT.

Source

Department of Signalling, The Babraham Institute, Cambridge, United Kingdom.

Abstract

Two highly similar, PtdIns(4,5)P2-selective, G beta gamma-activated PI3Ks were purified from pig neutrophil cytosol. Both were heterodimers, were composed of a 101 kDa protein and either a 120 kDa or a 117 kDa catalytic subunit, and were activated greater than 100-fold by G beta gammas. Peptide sequence-based oligonucleotide probes were used to clone cDNAs for the p120 and p101 species. The cDNA of p120 is highly related to p110 gamma, while the cDNA of p101 is not substantially related to anything in current databases. The proteins were expressed in and purified from insect and mammalian cells. They bound tightly to one another, both in vivo and in vitro, and in so doing, p101 amplified the effect of G beta gammas on the PI3K activity of p120 from less than 2-fold to greater than 100-fold.

PMID:: 9094719; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances, Secondary Source ID, Grant Support

Publication Types

MeSH Terms

Substances

Secondary Source ID

Grant Support

5 P30 CA08748/CA/NCI NIH HHS/United States

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Supplemental Content

Save items

Related citations in PubMed

Roles of G beta gamma in membrane recruitment and activation of p110 gamma/p101 phosphoinositide 3-kinase gamma. [J Cell Biol. 2003]
Roles of G beta gamma in membrane recruitment and activation of p110 gamma/p101 phosphoinositide 3-kinase gamma.
Brock C, Schaefer M, Reusch HP, Czupalla C, Michalke M, Spicher K, Schultz G, Nürnberg B. J Cell Biol. 2003 Jan 6; 160(1):89-99. Epub 2002 Dec 30.
Review A heterotrimeric GTPase-regulated isoform of PI3K and the regulation of its potential effectors. [Philos Trans R Soc Lond B Biol...]
Review A heterotrimeric GTPase-regulated isoform of PI3K and the regulation of its potential effectors.
Stephens L, Hawkins PT, Eguinoa A, Cooke F. Philos Trans R Soc Lond B Biol Sci. 1996 Feb 29; 351(1336):211-5.
[Search for a molecular determinant in phosphatidylinositol-3-kinase molecules, involved in the interaction with the beta-gamma-G protein dimer]. [Tsitologiia. 2002]
[Search for a molecular determinant in phosphatidylinositol-3-kinase molecules, involved in the interaction with the beta-gamma-G protein dimer].
Shpakov AO. Tsitologiia. 2002; 44(2):195-202.
Cloning and characterization of a G protein-activated human phosphoinositide-3 kinase. [Science. 1995]
Cloning and characterization of a G protein-activated human phosphoinositide-3 kinase.
Stoyanov B, Volinia S, Hanck T, Rubio I, Loubtchenkov M, Malek D, Stoyanova S, Vanhaesebroeck B, Dhand R, Nürnberg B. Science. 1995 Aug 4; 269(5224):690-3.
Review Synergistic activation of a family of phosphoinositide 3-kinase via G-protein coupled and tyrosine kinase-related receptors. [Chem Phys Lipids. 1999]
Review Synergistic activation of a family of phosphoinositide 3-kinase via G-protein coupled and tyrosine kinase-related receptors.
Katada T, Kurosu H, Okada T, Suzuki T, Tsujimoto N, Takasuga S, Kontani K, Hazeki O, Ui M. Chem Phys Lipids. 1999 Apr; 98(1-2):79-86.

See reviews... See all...

Cited by 77 PubMed Central articles

p110δ PI3 kinase pathway: emerging roles in cancer. [Front Oncol. 2013]
p110δ PI3 kinase pathway: emerging roles in cancer.
Tzenaki N, Papakonstanti EA. Front Oncol. 2013; 3:40. Epub 2013 Mar 1.
Nodal signaling regulates endodermal cell motility and actin dynamics via Rac1 and Prex1. [J Cell Biol. 2012]
Nodal signaling regulates endodermal cell motility and actin dynamics via Rac1 and Prex1.
Woo S, Housley MP, Weiner OD, Stainier DY. J Cell Biol. 2012 Sep 3; 198(5):941-52.
Cancer Therapy Targeting the HER2-PI3K Pathway: Potential Impact on the Heart. [Front Pharmacol. 2012]
Cancer Therapy Targeting the HER2-PI3K Pathway: Potential Impact on the Heart.
Klement GL, Goukassian D, Hlatky L, Carrozza J, Morgan JP, Yan X. Front Pharmacol. 2012; 3:113. Epub 2012 Jun 27.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
UniGene
UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records.
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.
Cited in Books
NCBI Bookshelf books that cite the current articles.

Recent activity

Clear Turn Off Turn On

The G beta gamma sensitivity of a PI3K is dependent upon a tightly associated ad...
The G beta gamma sensitivity of a PI3K is dependent upon a tightly associated adaptor, p101.
Cell. 1997 Apr 4 ;89(1):105-14.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: