Ultrastruct Pathol. 1997 Mar-Apr;21(2):183-93.

Identification of focal viral infections by confocal microscopy for subsequent ultrastructural analysis.

Miller SE, Levenson RM, Aldridge C, Hester S, Kenan DJ, Howell DN.

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Department of Microbiology, Duke University Medical Center, Durham, NC 27710, USA. saram@acpub.duke.edu

Abstract

A correlative microscopy method for the ultrastructural analysis of focal viral tissue infections is presented. Using a confocal scanning laser microscope, foci of infection are identified in tissue sections prior to embedment; a variety of techniques can be employed for viral detection, including staining with standard histochemical reagents and fluorescently labeled antibodies. Areas of infection identified using confocal microscopy are excised from the tissue sections, embedded, and examined by transmission electron microscopy. Applications of this technique in both diagnostic and basic research settings are described.

PMID:: 9090029; [PubMed - indexed for MEDLINE]

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Identification of focal viral infections by confocal microscopy for subsequent ultrastructural analysis.

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