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An mRNA encoding a putative GABA-gated chloride channel is expressed in the human cardiac conduction system.
Laboratoire de Neurophysiologie, Université de Bordeaux 2, France.
GABA-gated chloride channels are the main inhibitory neurotransmitter receptors in the CNS. Conserved domains among members of previously described GABAA receptor subunits were used to design degenerate sense and antisense oligonucleotides. A PCR product from this amplification was used to isolate a full-length cDNA. The predicted protein has many of the features shared by other members of the ligand-gated ion channel family. This channel subunit has significant amino acid identity (25-40%) with members of GABAA and GABAC receptor subunits and thus may represent a new subfamily of the GABA receptor channel. Although we cannot rule out that this clone encodes a receptor for an unidentified ligand, it was termed GABA chi. This gene is mainly expressed in placenta and in heart; however, placenta appears to express only an unspliced mRNA. In situ hybridization reveals that the GABA chi subunit mRNA is present in the electrical conduction system of the human heart. Our results suggest that novel GABA receptors expressed outside of the CNS may regulate cardiac function.
PMID: 9084408 [PubMed - indexed for MEDLINE]
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Cited by 3 PubMed Central articles
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Investigation of gamma-aminobutyric acid (GABA) A receptors genes and migraine susceptibility.
Fernandez F, Esposito T, Lea RA, Colson NJ, Ciccodicola A, Gianfrancesco F, Griffiths LR.
BMC Med Genet. 2008 Dec 16; 9:109. Epub 2008 Dec 16.
[BMC Med Genet. 2008]
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Positive allosteric modulation by ultraviolet irradiation on GABA(A), but not GABA(C), receptors expressed in Xenopus oocytes.
Chang Y, Xie Y, Weiss DS.
J Physiol. 2001 Oct 15; 536(Pt 2):471-8.
[J Physiol. 2001]
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Allosteric activation mechanism of the alpha1beta2gamma2 gamma-aminobutyric acid type A receptor revealed by mutation of the conserved M2 leucine
Chang Y, Weiss DS.
Biophys J. 1999 Nov; 77(5):2542-51.
[Biophys J. 1999]