Abstract
The temporal distribution of ATP/citrate lyase (ACL) activity in developing seeds of Brassica napus L. closely paralleled both that of acetyl-CoA carboxylase and the overall rate of lipid biosynthesis. Maximum ACL activities (250 nmol acetyl-CoA formed min-1.g fresh seed) were recorded between 35 to 42 d after pollination and, if the in vitro data could be extrapolated to the situation in vivo, could account for half of the acetyl-CoA required for the measured rate of fatty acid biosynthesis during seed development. The enzyme appeared to be localized in a subcellular compartment, which was clearly separated from mitochondria on a sucrose gradient and by differential centrifugation, and which corresponded to the chloroplast organelle.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP Citrate (pro-S)-Lyase / metabolism*
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Acetates / metabolism
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Acetyl-CoA Carboxylase / metabolism
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Brassica / enzymology*
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Cell Fractionation
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Centrifugation, Density Gradient
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Chloroplasts / enzymology
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Chloroplasts / metabolism
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Citric Acid / metabolism
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Cytosol / enzymology
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Cytosol / metabolism
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Fumarate Hydratase / metabolism
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Glucosephosphate Dehydrogenase / metabolism
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Hydrogen-Ion Concentration
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Lipid Metabolism*
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Lipids / biosynthesis
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Malate Dehydrogenase / metabolism
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Mitochondria / enzymology
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Mitochondria / metabolism
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Plant Oils / metabolism
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Seeds / enzymology
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Seeds / metabolism
Substances
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Acetates
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Lipids
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Plant Oils
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Citric Acid
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Malate Dehydrogenase
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malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)
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Glucosephosphate Dehydrogenase
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ATP Citrate (pro-S)-Lyase
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Fumarate Hydratase
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Acetyl-CoA Carboxylase