Structure determination of the O-antigenic polysaccharide from the enterotoxigenic Escherichia coli (ETEC) O101

M Staaf; F Urbina; A Weintraub; G Widmalm

doi:10.1016/s0008-6215(96)00274-1

Structure determination of the O-antigenic polysaccharide from the enterotoxigenic Escherichia coli (ETEC) O101

Carbohydr Res. 1997 Jan 17;297(3):297-9. doi: 10.1016/s0008-6215(96)00274-1.

Authors

M Staaf¹, F Urbina, A Weintraub, G Widmalm

Affiliation

¹ Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden.

PMID: 9060191
DOI: 10.1016/s0008-6215(96)00274-1

Abstract

The O-antigenic polysaccharide of the lipopolysaccharide from the enterotoxigenic Escherichia coli O101 has been investigated. The composition and sequence of the repeating units was established by sugar and methylation analysis together with 1H and 13C NMR spectroscopy. The sequence was corroborated using the computer program CASPER. The structure of the repeating unit of the polysaccharide from E. coli O101 is as follows: -->6)-alpha-D-GlcpNAc-1-->4-alpha-D-GalpNAc-(1-->.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Conformation
Carbohydrate Sequence
Enterotoxins / biosynthesis
Escherichia coli / immunology*
Escherichia coli / pathogenicity
Magnetic Resonance Spectroscopy
Molecular Sequence Data
O Antigens / chemistry*
Oligosaccharides / chemistry*
Polysaccharides, Bacterial / chemistry
Polysaccharides, Bacterial / isolation & purification
Serotyping

Substances

Enterotoxins
O Antigens
Oligosaccharides
Polysaccharides, Bacterial