The purpose of the study was to determine whether antigenic differences occurred in four stabilates of Babesia bigemina derived from a single purified isolate and propagated as acute and chronic, blood-borne and tick-borne infections in Colombian cattle. Antigens were characterised by means of the complement fixation (CF), gel diffusion (GD), agar gel electrophoresis (AGE) and the indirect haemagglutination tests (IHA). Differences were detected. Acute blood and chronic blood antigens were similar, as were acute tick and chronic tick antigens, when compared by IHA and GD. Similarities were observed between acute blood anc acute tick and between chronic blood and chronic tick when these antigens were compared by AGE and CF.