Nature. 1997 Feb 13;385(6617):595-602.

Three-dimensional structure of the tyrosine kinase c-Src.

Source

Laboratory of Molecular Medicine, Children's Hospital, Boston, Massachusetts 02115, USA.

Abstract

The structure of a large fragment of the c-Src tyrosine kinase, comprising the regulatory and kinase domains and the carboxy-terminal tall, has been determined at 1.7 A resolution in a closed, inactive state. Interactions among domains, stabilized by binding of the phosphorylated tail to the SH2 domain, lock the molecule in a conformation that simultaneously disrupts the kinase active site and sequesters the binding surfaces of the SH2 and SH3 domains. The structure shows how appropriate cellular signals, or transforming mutations in v-Src, could break these interactions to produce an open, active kinase.

Comment in

Nature. 1997 Feb 13;385(6617):582-3, 585.

PMID:: 9024657; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances, Secondary Source ID

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

Proto-Oncogene Proteins pp60(c-src)

Secondary Source ID

PDB/1FMK

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Molecular Biology Databases

Saccharomyces Genome Database

Supplemental Content

Save items

Related citations in PubMed

The 2.35 A crystal structure of the inactivated form of chicken Src: a dynamic molecule with multiple regulatory interactions. [J Mol Biol. 1997]
The 2.35 A crystal structure of the inactivated form of chicken Src: a dynamic molecule with multiple regulatory interactions.
Williams JC, Weijland A, Gonfloni S, Thompson A, Courtneidge SA, Superti-Furga G, Wierenga RK. J Mol Biol. 1997 Dec 19; 274(5):757-75.
Structure of the regulatory domains of the Src-family tyrosine kinase Lck. [Nature. 1994]
Structure of the regulatory domains of the Src-family tyrosine kinase Lck.
Eck MJ, Atwell SK, Shoelson SE, Harrison SC. Nature. 1994 Apr 21; 368(6473):764-9.
Review Structures of Src-family tyrosine kinases. [Curr Opin Struct Biol. 1997]
Review Structures of Src-family tyrosine kinases.
Sicheri F, Kuriyan J. Curr Opin Struct Biol. 1997 Dec; 7(6):777-85.
Structure of the carboxyl-terminal Src kinase, Csk. [J Biol Chem. 2002]
Structure of the carboxyl-terminal Src kinase, Csk.
Ogawa A, Takayama Y, Sakai H, Chong KT, Takeuchi S, Nakagawa A, Nada S, Okada M, Tsukihara T. J Biol Chem. 2002 Apr 26; 277(17):14351-4. Epub 2002 Mar 7.
Review Biochemistry of the Src protein-tyrosine kinase: regulation by SH2 and SH3 domains. [Recent Prog Horm Res. 1994]
Review Biochemistry of the Src protein-tyrosine kinase: regulation by SH2 and SH3 domains.
Liu X, Pawson T. Recent Prog Horm Res. 1994; 49:149-60.

See reviews... See all...

Cited by over 100 PubMed Central articles

SH3 Domain Tyrosine Phosphorylation - Sites, Role and Evolution. [PLoS One. 2012]
SH3 Domain Tyrosine Phosphorylation - Sites, Role and Evolution.
Tatárová Z, Brábek J, Rösel D, Novotný M. PLoS One. 2012; 7(5):e36310. Epub 2012 May 15.
HIF-1 and c-Src mediate increased glucose uptake induced by endothelin-1 and connexin43 in astrocytes. [PLoS One. 2012]
HIF-1 and c-Src mediate increased glucose uptake induced by endothelin-1 and connexin43 in astrocytes.
Valle-Casuso JC, González-Sánchez A, Medina JM, Tabernero A. PLoS One. 2012; 7(2):e32448. Epub 2012 Feb 23.
Mutations in the catalytic loop HRD motif alter the activity and function of Drosophila Src64. [PLoS One. 2011]
Mutations in the catalytic loop HRD motif alter the activity and function of Drosophila Src64.
Strong TC, Kaur G, Thomas JH. PLoS One. 2011; 6(11):e28100. Epub 2011 Nov 23.

See all...

Structures reported by this article

Crystal Structure Of Human Tyrosine-Protein Kinase C-Src

PDB: 1FMK

Source: Homo sapiens

Method: X-Ray Diffraction

Resolution: 1.5 Å

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
HomoloGene
HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Protein Clusters
Clusters of related proteins from the Protein Clusters database that cite the current articles. Sources of references in Protein Clusters include the associated Gene and Conserved Domain records as well as NCBI added citations.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
UniGene
UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records.
Domains
Conserved Domain Database (CDD) records that cite the current articles. Citations are from the CDD source database records (PFAM, SMART).
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Structure
Three-dimensional structure records in the NCBI Structure database for data reported in the current articles.
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.
Cited in Books
NCBI Bookshelf books that cite the current articles.

Recent activity

Clear Turn Off Turn On

Three-dimensional structure of the tyrosine kinase c-Src.
Three-dimensional structure of the tyrosine kinase c-Src.
Nature. 1997 Feb 13 ;385(6617):595-602.

PubMed
Twenty-four-hour profiles of luteinizing hormone, follicle-stimulating hormone, ...
Twenty-four-hour profiles of luteinizing hormone, follicle-stimulating hormone, testosterone, and estradiol levels: a semilongitudinal study throughout puberty in healthy boys.
J Clin Endocrinol Metab. 1997 Feb ;82(2):541-9.

PubMed
Testosterone replacement increases fat-free mass and muscle size in hypogonadal ...
Testosterone replacement increases fat-free mass and muscle size in hypogonadal men.
J Clin Endocrinol Metab. 1997 Feb ;82(2):407-13.

PubMed
Optimization of the detection of bacteriophages induced from Listeria sp.
Optimization of the detection of bacteriophages induced from Listeria sp.
Lett Appl Microbiol. 1997 Jan ;24(1):51-4.

PubMed
Novel group within the kingdom Crenarchaeota from boreal forest soil.
Novel group within the kingdom Crenarchaeota from boreal forest soil.
Appl Environ Microbiol. 1997 Feb ;63(2):803-5.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: