Your browser version may not work well with NCBI's Web applications. More information here...
1: Nucleic Acids Res. 1997 Feb 15;25(4):877-82.Click here to read Click here to read Links

Phosphorylation of ATF-1 enhances its DNA binding and transcription of the Na,K-ATPase alpha 1 subunit gene promoter.

Kobayashi M, Shimomura A, Hagiwara M, Kawakami K.

Department of Biology, Jichi Medical School, Tochigi, Japan.

Transcriptional activity of both ATF-1 and CREB is enhanced by protein phosphorylation. While enhancement has been attributed to an increase in binding affinity for a co-activator (CBP), induction of the DNA binding activity by phosphorylation is an open question. Using the Na,K-ATPase alpha1 subunit gene promoter, which has an asymmetrical ATF/CRE site, we analyzed the effect of phosphorylation on DNA binding activity of the ATF-1-CREB heterodimer. Dephosphorylation of the heterodimer in nuclear extracts reduced binding to the ATF/CRE site. Phosphorylation of ATF-1 at Ser63 enhanced its binding to the ATF/CRE site in both the homodimeric and heterodimeric forms. Transcription of the Na,K-ATPase alpha 1 subunit gene promoter was also stimulated by phosphorylated ATF-1 in vitro.

PMID: 9016641 [PubMed - indexed for MEDLINE]

PMCID: PMC146500

Patient Drug Information

  • Potassium (Glu-K® , K+ 10® , K+ 8® , ...)

    Potassium is essential for the proper functioning of the heart, kidneys, muscles, nerves, and digestive system. Usually the food you eat supplies all of the potassium you need. However, certain diseases (e.g., kidney dis...

  • Source: AHFS Consumer Medication Information