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Nucleic Acids Res. 1997 Jan 15;25(2):451-2.

Recombination-mediated PCR-directed plasmid construction in vivo in yeast.

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  • 1Affymax Research Institute, 4001 Miranda Avenue, Palo Alto, CA 94304, USA.

Abstract

We have extended the technique of PCR-directed recombination in Saccharomyces cerevisiae to develop a simple method for plasmid or gene construction in the absence of suitable restriction sites. The DNA to be cloned is PCR-amplified with 30-40 bp of homology to a linearized yeast plasmid. Co-transformation into yeast results in homologous recombination at a position directed by the PCR oligonucleotides.

PMID:
9016579
[PubMed - indexed for MEDLINE]
PMCID:
PMC146432
Free PMC Article
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