J Clin Microbiol. 1997 Feb;35(2):492-4.

Mutations in the rpoB gene of Mycobacterium tuberculosis that interfere with PCR-single-strand conformation polymorphism analysis for rifampin susceptibility testing.

Kim BJ, Kim SY, Park BH, Lyu MA, Park IK, Bai GH, Kim SJ, Cha CY, Kook YH.

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Department of Microbiology and Cancer Research Center, Seoul National University College of Medicine, Korea.

Abstract

Rifampin susceptibility of 32 rifampin-resistant and 26 rifampin-susceptible Mycobacterium tuberculosis strains was analyzed by PCR-single-strand conformation polymorphism (SSCP) and DNA sequencing within the 157-bp region of the rpoB gene (Ala500 to Val550). Two false-positive PCR-SSCP results were observed among the susceptible strains due to the silent mutation Gln513 (CAA-->CAG) and the deletion mutation Thr508 and Ser509. Another silent mutation [Leu511 (CTG-->CTA)], combined with the mutation Ser531-->Leu, was observed in a resistant strain. These results suggest that to rule out false-positive PCR-SSCP results, sequencing of the target DNA is required.

PMID:: 9003625; [PubMed - indexed for MEDLINE]
PMCID:: PMC229609

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