Format

Send to

Choose Destination
See comment in PubMed Commons below
Methods. 1997 Feb;11(2):215-24.

Measurement of in vivo DNA binding by sequence-specific transcription factors using UV cross-linking.

Author information

  • 1Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06520-208114, USA.

Abstract

This paper describes an in vivo UV cross-linking protocol that is sensitive enough to detect DNA binding by sequence-specific transcription factors in Drosophila embryos and tissue culture cells. The strength of this approach is that it provides a quantitative measure of DNA binding in vivo with unambiguous identification of the factor involved in the binding. This assay often detects DNA binding properties of proteins that were not predicted from previous experiments, and it can be used to directly test diverse models of gene regulation in the context of a living organism.

[PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Other Literature Sources

Molecular Biology Databases

PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk