Nat Struct Biol. 1997 Jan;4(1):64-9.

The structure of MCP-1 in two crystal forms provides a rare example of variable quaternary interactions.

Lubkowski J, Bujacz G, Boqué L, Domaille PJ, Handel TM, Wlodawer A.

Source

Macromolecular Structure Laboratory, NCI-Frederick Cancer Research and Development Center, ABL-Basic Research Program, Maryland 21702, USA.

Abstract

The X-ray crystal structure of recombinant human monocyte chemoattractant protein (MCP-1) has been solved in two crystal forms. One crystal form (P), refined to 1.85 A resolution, contains a dimer in the asymmetric unit, while the other (I) contains a monomer and was refined at 2.4 A. Although both crystal forms grow together in the same droplet, the respective quaternary structures of the protein differ dramatically. In addition, both X-ray structures differ to a similar extent from the solution structure of MCP-1. Such extent of variability of quaternary structures is unprecedented. In the crystal structures, the well-ordered N termini of MCP-1 form 3(10)-helices. Comparison of the three MCP-1 structures revealed a direct correlation between the main-chain conformation of the first two cysteine residues and the quaternary arrangements. These data can be used to explain the structural basis for the assignment of residues responsible for biological activity.

PMID:: 8989326; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, U.S. Gov't, P.H.S.

MeSH Terms

Substances

Supplemental Content

Save items

Related citations in PubMed

Synthesis by native chemical ligation and crystal structure of human CCL2. [Biopolymers. 2010]
Synthesis by native chemical ligation and crystal structure of human CCL2.
Grygiel TL, Teplyakov A, Obmolova G, Stowell N, Holland R, Nemeth JF, Pomerantz SC, Kruszynski M, Gilliland GL. Biopolymers. 2010; 94(3):350-9.
Nerve growth factor in different crystal forms displays structural flexibility and reveals zinc binding sites. [J Mol Biol. 1994]
Nerve growth factor in different crystal forms displays structural flexibility and reveals zinc binding sites.
Holland DR, Cousens LS, Meng W, Matthews BW. J Mol Biol. 1994 Jun 10; 239(3):385-400.
Crystal structure of native and Cd/Cd-substituted Dioclea guianensis seed lectin. A novel manganese-binding site and structural basis of dimer-tetramer association. [J Mol Biol. 2001]
Crystal structure of native and Cd/Cd-substituted Dioclea guianensis seed lectin. A novel manganese-binding site and structural basis of dimer-tetramer association.
Wah DA, Romero A, Gallego del Sol F, Cavada BS, Ramos MV, Grangeiro TB, Sampaio AH, Calvete JJ. J Mol Biol. 2001 Jul 20; 310(4):885-94.
Structure of human monocyte chemoattractant protein 4 (MCP-4/CCL13). [Acta Crystallogr D Biol Crysta...]
Structure of human monocyte chemoattractant protein 4 (MCP-4/CCL13).
Barinka C, Prahl A, Lubkowski J. Acta Crystallogr D Biol Crystallogr. 2008 Mar; 64(Pt 3):273-8. Epub 2008 Feb 20.
Review Structures of membrane proteins determined at atomic resolution. [J Biochem. 1998]
Review Structures of membrane proteins determined at atomic resolution.
Sakai H, Tsukihara T. J Biochem. 1998 Dec 1; 124(6):1051-9.

See reviews... See all...

Cited by 16 PubMed Central articles

NMR in the Analysis of Functional Chemokine Interactions and Drug Discovery. [Drug Discov Today Technol. 2012]
NMR in the Analysis of Functional Chemokine Interactions and Drug Discovery.
Ziarek JJ, Volkman BF. Drug Discov Today Technol. 2012; 9(4):e293-e299.
Review Chemokine oligomerization and interactions with receptors and glycosaminoglycans: the role of structural dynamics in function. [Exp Cell Res. 2011]
Review Chemokine oligomerization and interactions with receptors and glycosaminoglycans: the role of structural dynamics in function.
Salanga CL, Handel TM. Exp Cell Res. 2011 Mar 10; 317(5):590-601. Epub 2011 Jan 9.
Characterization of the chemokine CXCL11-heparin interaction suggests two different affinities for glycosaminoglycans. [J Biol Chem. 2010]
Characterization of the chemokine CXCL11-heparin interaction suggests two different affinities for glycosaminoglycans.
Severin IC, Gaudry JP, Johnson Z, Kungl A, Jansma A, Gesslbauer B, Mulloy B, Power C, Proudfoot AE, Handel T. J Biol Chem. 2010 Jun 4; 285(23):17713-24. Epub 2010 Apr 2.

See all...

Structures reported by this article

Monocyte Chemoattractant Protein 1, I-Form

PDB: 1DOL

Source: Homo sapiens

Method: X-Ray Diffraction

Resolution: 2.4 Å

See all 2 structures...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
Gene (GeneRIF)
Gene records that have the current articles as Reference into Function citations (GeneRIFs). NLM staff reviewing the literature while indexing MEDLINE add GeneRIFs manually.
HomoloGene
HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
UniGene
UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records.
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Structure
Three-dimensional structure records in the NCBI Structure database for data reported in the current articles.
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

The structure of MCP-1 in two crystal forms provides a rare example of variable ...
The structure of MCP-1 in two crystal forms provides a rare example of variable quaternary interactions.
Nat Struct Biol. 1997 Jan ;4(1):64-9.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: