Microbiology. 1996 Nov;142 ( Pt 11):3219-30.

The purB gene of Escherichia coli K-12 is located in an operon.

Green SM, Malik T, Giles IG, Drabble WT.

Source

Department of Biochemistry, University of Southampton, Crescent East, UK.

Abstract

The structural gene (purB) for succinyl-AMP (S-AMP) lyase and three additional ORFs are on the same DNA strand of the chromosome of Escherichia coli. Cassette mutagenesis and primer extension mapping demonstrated that purB is co-transcribed with an upstream gene (ORF23, or ycfC) encoding a 22.9 kDa membrane-associated protein of non-essential, but unknown, function unrelated to purine biosynthesis. The purB operon lies between phoP and an ORF expressing an essential function which may correspond to asuE (trmU). S-AMP lyase was purified to near homogeneity. The purified enzyme is a homotetramer of 50 kDa subunits, has a K(m) for S-AMP of 3.7 microM and a pH optimum of 7.4-7.6.

PMID:: 8969519; [PubMed - indexed for MEDLINE]

Free full text

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Adenylosuccinate lyase of Bacillus subtilis regulates the activity of the glutamyl-tRNA synthetase. [Proc Natl Acad Sci U S A. 1992]
Adenylosuccinate lyase of Bacillus subtilis regulates the activity of the glutamyl-tRNA synthetase.
Gendron N, Breton R, Champagne N, Lapointe J. Proc Natl Acad Sci U S A. 1992 Jun 15; 89(12):5389-92.
Escherichia coli purB gene: cloning, nucleotide sequence, and regulation by purR. [J Bacteriol. 1992]
Escherichia coli purB gene: cloning, nucleotide sequence, and regulation by purR.
He B, Smith JM, Zalkin H. J Bacteriol. 1992 Jan; 174(1):130-6.
Cloning of a cDNA encoding adenylosuccinate lyase by functional complementation in Escherichia coli. [J Biol Chem. 1990]
Cloning of a cDNA encoding adenylosuccinate lyase by functional complementation in Escherichia coli.
Aimi J, Badylak J, Williams J, Chen ZD, Zalkin H, Dixon JE. J Biol Chem. 1990 Jun 5; 265(16):9011-4.
Review Genetic switches by DNA inversions in prokaryotes. [Biochim Biophys Acta. 1984]
Review Genetic switches by DNA inversions in prokaryotes.
Plasterk RH, Van de Putte P. Biochim Biophys Acta. 1984 Jun 16; 782(2):111-9.
Review The two umuDC-like operons, samAB and umuDCST, in Salmonella typhimurium: the umuDCST operon may reduce UV-mutagenesis-promoting ability of the samAB operon. [Basic Life Sci. 1993]
Review The two umuDC-like operons, samAB and umuDCST, in Salmonella typhimurium: the umuDCST operon may reduce UV-mutagenesis-promoting ability of the samAB operon.
Nohmi T, Hakura A, Nakai Y, Watanabe M, Yamada M, Murayama SY, Sofuni T. Basic Life Sci. 1993; 61:247-55.

See reviews... See all...

Cited by 8 PubMed Central articles

Review Bioinformatics resources for the study of gene regulation in bacteria. [J Bacteriol. 2009]
Review Bioinformatics resources for the study of gene regulation in bacteria.
Collado-Vides J, Salgado H, Morett E, Gama-Castro S, Jiménez-Jacinto V, Martínez-Flores I, Medina-Rivera A, Muñiz-Rascado L, Peralta-Gil M, Santos-Zavaleta A. J Bacteriol. 2009 Jan; 191(1):23-31. Epub 2008 Oct 31.
Review On the origin of biochemistry at an alkaline hydrothermal vent. [Philos Trans R Soc Lond B Biol...]
Review On the origin of biochemistry at an alkaline hydrothermal vent.
Martin W, Russell MJ. Philos Trans R Soc Lond B Biol Sci. 2007 Oct 29; 362(1486):1887-925.
Transcriptional response of Escherichia coli to TPEN. [J Bacteriol. 2006]
Transcriptional response of Escherichia coli to TPEN.
Sigdel TK, Easton JA, Crowder MW. J Bacteriol. 2006 Sep; 188(18):6709-13.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
BioSystems
Pathways and biological systems (BioSystems) that cite the current articles. Citations are from the BioSystems source databases (KEGG and BioCyc).
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Protein Clusters
Clusters of related proteins from the Protein Clusters database that cite the current articles. Sources of references in Protein Clusters include the associated Gene and Conserved Domain records as well as NCBI added citations.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
Domains
Conserved Domain Database (CDD) records that cite the current articles. Citations are from the CDD source database records (PFAM, SMART).
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

The purB gene of Escherichia coli K-12 is located in an operon.
The purB gene of Escherichia coli K-12 is located in an operon.
Microbiology. 1996 Nov ;142 ( Pt 11):3219-30.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

The purB gene of Escherichia coli K-12 is located in an operon.

Source

Abstract

Publication Types, MeSH Terms, Substances, Secondary Source ID

Publication Types

MeSH Terms

Substances

Secondary Source ID

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Cited by 8 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information