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J Bacteriol. 1996 Dec;178(24):7311-5.

13C nuclear magnetic resonance analysis of glucose and citrate end products in an ldhL-ldhD double-knockout strain of Lactobacillus plantarum.

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  • 1Laboratoire de Génétique Moléculaire, Unité de Génétique, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.

Abstract

We have examined the metabolic consequences of knocking out the two ldh genes in Lactobacillus plantarum using 13C nuclear magnetic resonance. Unlike its wild-type isogenic progenitor, which produced lactate as the major metabolite under all conditions tested, ldh null strain TF103 mainly produced acetoin. A variety of secondary end products were also found, including organic acids (acetate, succinate, pyruvate, and lactate), ethanol, 2,3-butanediol, and mannitol.

PMID:
8955418
[PubMed - indexed for MEDLINE]
PMCID:
PMC178649
Free PMC Article
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