Abstract
Cpr6 and Cpr7, the Saccharomyces cerevisiae homologs of cyclophilin-40 (CyP-40), were shown to form complexes with Hsp90, a protein chaperone that functions in several signal transduction pathways. Deletion of CPR7 caused severe growth defects when combined with mutations that decrease the amount of Hsp90 or Sti1, another component of the Hsp90 chaperone machinery. The activities of two heterologous Hsp90-dependent signal transducers expressed in yeast, glucocorticoid receptor and pp60(v-src) kinase, were adversely affected by cpr7 null mutations. These results suggest that CyP-40 cyclophilins play a general role in Hsp90-dependent signal transduction pathways under normal growth conditions.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Isomerases / genetics
-
Amino Acid Isomerases / metabolism
-
Amino Acid Isomerases / physiology*
-
Carrier Proteins / genetics
-
Carrier Proteins / metabolism
-
Carrier Proteins / physiology*
-
Cyclophilins*
-
Fungal Proteins / genetics
-
Fungal Proteins / metabolism
-
Fungal Proteins / physiology*
-
HSP90 Heat-Shock Proteins / genetics
-
HSP90 Heat-Shock Proteins / metabolism
-
HSP90 Heat-Shock Proteins / physiology*
-
Heat-Shock Proteins
-
Molecular Chaperones / genetics
-
Molecular Chaperones / metabolism
-
Molecular Chaperones / physiology*
-
Mutation
-
Oncogene Protein pp60(v-src) / metabolism
-
Peptidyl-Prolyl Isomerase F
-
Peptidylprolyl Isomerase*
-
Proto-Oncogene Proteins pp60(c-src) / metabolism
-
Receptors, Glucocorticoid / metabolism
-
Saccharomyces cerevisiae / genetics
-
Saccharomyces cerevisiae / growth & development
-
Saccharomyces cerevisiae / metabolism
-
Saccharomyces cerevisiae / physiology*
-
Saccharomyces cerevisiae Proteins
-
Signal Transduction*
Substances
-
CPR6 protein, S cerevisiae
-
CPR7 protein, S cerevisiae
-
Carrier Proteins
-
Peptidyl-Prolyl Isomerase F
-
Fungal Proteins
-
HSP90 Heat-Shock Proteins
-
Heat-Shock Proteins
-
Molecular Chaperones
-
Receptors, Glucocorticoid
-
STI1 protein, S cerevisiae
-
Saccharomyces cerevisiae Proteins
-
Oncogene Protein pp60(v-src)
-
Proto-Oncogene Proteins pp60(c-src)
-
Amino Acid Isomerases
-
Cyclophilins
-
Peptidylprolyl Isomerase