Molecular Genetics Group, John Curtin School of Medical Research, Australian National University, PO Box 334, Canberra, ACT 2601, Australia. Rohan.Baker@anu.edu.au
Abstract
Expressing proteins and polypeptides as fusions to ubiquitin offers the advantage of an often dramatic increase in yield, and the ability to produce any desired amino-terminal residue upon ubiquitin cleavage. The recent availability of cloned ubiquitin-cleaving enzymes has enhanced this technique for both bacterial and eukaryotic host systems.