Investigation of the chemical stability of a new growth hormone-releasing hormone (GHRH) analogue by HPLC

M Idei; I Mezö; E Z Szabó; G Kéri

doi:10.1002/(SICI)1099-0801(199603)10:2<89::AID-BMC560>3.0.CO;2-Q

Investigation of the chemical stability of a new growth hormone-releasing hormone (GHRH) analogue by HPLC

Biomed Chromatogr. 1996 Mar-Apr;10(2):89-91. doi: 10.1002/(SICI)1099-0801(199603)10:2<89::AID-BMC560>3.0.CO;2-Q.

Authors

M Idei¹, I Mezö, E Z Szabó, G Kéri

Affiliation

¹ I. Institute of Biochemistry, Semmelweis University, Medical School, Budapest, Hungary.

PMID: 8924733
DOI: 10.1002/(SICI)1099-0801(199603)10:2<89::AID-BMC560>3.0.CO;2-Q

Abstract

The stability of a new active growth hormone-releasing hormone analogue (D-Ala2,Nle27,(gamma-amino-butyric acid)30-GHRH(1-30)-NH2) was investigated during storage at different temperatures in aqueous solution. Samples stored for various periods of time were analysed by HPLC. It is concluded that in aqueous solution D-Ala2, Nle27,(gamma-amino-butyric acid)30-growth hormone-releasing hormone (1-30)-NH2 is stable: at least for 36 days at 4 degrees C; for 28 days at 25 degrees C; and for 10 days at 37 degrees C.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Chromatography, High Pressure Liquid
Drug Stability
Growth Hormone-Releasing Hormone / analogs & derivatives*
Growth Hormone-Releasing Hormone / chemistry*
Molecular Sequence Data
Solutions
Temperature

Substances

Solutions
somatotropin-releasing hormone-(1-30)-amide, Ala(2)-Nle(27)-GABA(30)-
Growth Hormone-Releasing Hormone