Antimicrob Agents Chemother. 1996 Nov;40(11):2651-4.

Quantitation of intracellular zidovudine phosphates by use of combined cartridge-radioimmunoassay methodology.

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Department of Infectious Disease, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.

Abstract

This report describes the development of a potentially clinical method to measure the cellular metabolites of zidovudine (ZDV) in patients receiving the drug. This new method combines the use of Sep-Pak cartridges to separate ZDV phosphates with radioimmunoassaying to quantitate ZDV. The detection limit is 0.02 pmol/10(6) cells, and this assay can measure a wide range of intracellular drug concentrations. The use of the cartridge-radioimmunoassay methodology should prove very useful for in vivo cellular pharmacokinetic studies of ZDV.

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PMCID: PMC163594

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Quantitation of intracellular zidovudine phosphates by use of combined cartridge-radioimmunoassay methodology.

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