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J Bacteriol. 1996 Nov;178(21):6357-60.

Apramycin resistance as a selective marker for gene transfer in mycobacteria.

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  • 1Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada.

Abstract

We have explored the potential of using the apramycin resistance gene as a marker in mycobacterial gene transfer studies. Shuttle plasmids available for both electroporation and conjugation studies have been constructed, and we have successfully validated the use of the apramycin resistance gene as a component of cloning vectors for Mycobacterium smegmatis, M. bovis BCG, and M. tuberculosis.

PMID:
8892841
PMCID:
PMC178512
[PubMed - indexed for MEDLINE]
Free PMC Article
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