A specific high-affinity binding macromolecule for 1,25-dihydroxyvitamin D3 in fetal bone

Science. 1977 Sep 9;197(4308):1086-8. doi: 10.1126/science.887939.

Abstract

Cytosol fractions were prepared from fetal rat or embryonic chick calvaria and analyzed for binding of vitamin D3 metabolites on sucrose density gradients. Both cytosol fractions contain a 3.5S macromolecule which specifically binds 1,25-dihydroxy-[3H]vitamin D3 and in addition, a 5 to 6S macromolecule which binds 25-hydroxy-[3H]vitamin D3. In rat calvaria cytosol, 1,25-dihydroxy-[3H]vitamin D3 also binds to the 5 to 6S macromolecule but appears to have greater affinity for the 3.5S component.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bone and Bones / embryology*
  • Bone and Bones / metabolism
  • Carrier Proteins / metabolism*
  • Chick Embryo
  • Cytosol / metabolism
  • Dihydroxycholecalciferols / metabolism*
  • Hydroxycholecalciferols / metabolism*
  • In Vitro Techniques
  • Rats
  • Receptors, Steroid

Substances

  • Carrier Proteins
  • Dihydroxycholecalciferols
  • Hydroxycholecalciferols
  • Receptors, Steroid