Abstract
129/Sv mice are resistant to induction of experimental autoimmune encephalomyelitis (EAE) induced with myelin oligodendrocyte glycoprotein peptide (MOG35-55). Mice of this strain lacking the gene coding for the ligand-binding chain of the IFN-gamma receptor develop EAE with high morbidity and mortality. Spleen cells from sensitized IFN-gammaR-/- mice proliferated extensively when stimulated with MOG peptide in culture and produced high levels of IFN-gamma and TNF but no detectable IL-4. Transfer of spleen cells from sensitized IFN-gammaR-/- mice produced EAE in both IFN-gammaR+/+ and IFN-gammaR-/- recipients. Disease was severe in IFN-gammaR-/- recipients and mortality high (77%). Surviving mice remained moribund until termination of the experiments. IFN-gammaR+/+ recipients developed disease of equal severity, but with no mortality, and recovered significantly. These results indicate that IFN-gamma is not essential for the generation or function of anti-MOG35-55 effector cells but does play an important role in down-regulating EAE at both the effector and induction phase of disease.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, CD / genetics
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Antigens, CD / metabolism
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Antigens, Surface
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Autoantibodies / biosynthesis
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Cytokines / biosynthesis
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Down-Regulation
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Encephalomyelitis, Autoimmune, Experimental / etiology*
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Encephalomyelitis, Autoimmune, Experimental / genetics
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Encephalomyelitis, Autoimmune, Experimental / immunology
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Female
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Interferon gamma Receptor
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Interferon-gamma / genetics
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Interferon-gamma / physiology*
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Lymphocyte Activation
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Male
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Mice
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Mice, Knockout
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Molecular Sequence Data
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Myelin Proteins
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Myelin-Associated Glycoprotein / genetics
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Myelin-Associated Glycoprotein / immunology*
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Myelin-Oligodendrocyte Glycoprotein
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Oligodendroglia / immunology
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Peptide Fragments / genetics
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Peptide Fragments / immunology
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Receptors, Interferon / genetics
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Receptors, Interferon / metabolism
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Spinal Cord / pathology
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Spleen / immunology
Substances
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Antigens, CD
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Antigens, Surface
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Autoantibodies
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Cytokines
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Mog protein, mouse
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Myelin Proteins
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Myelin-Associated Glycoprotein
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Myelin-Oligodendrocyte Glycoprotein
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Peptide Fragments
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Receptors, Interferon
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Interferon-gamma