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Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):10614-9.

Parallel human genome analysis: microarray-based expression monitoring of 1000 genes.

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  • 1Department of Biochemistry, Beckman Center, Stanford University Medical Center, CA 94305, USA. schena@cmgm.stanford.edu

Abstract

Microarrays containing 1046 human cDNAs of unknown sequence were printed on glass with high-speed robotics. These 1.0-cm2 DNA "chips" were used to quantitatively monitor differential expression of the cognate human genes using a highly sensitive two-color hybridization assay. Array elements that displayed differential expression patterns under given experimental conditions were characterized by sequencing. The identification of known and novel heat shock and phorbol ester-regulated genes in human T cells demonstrates the sensitivity of the assay. Parallel gene analysis with microarrays provides a rapid and efficient method for large-scale human gene discovery.

PMID:
8855227
[PubMed - indexed for MEDLINE]
PMCID:
PMC38202
Free PMC Article
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