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Exp Clin Immunogenet. 1996;13(1):30-5.

An improved method for the detection of peptide-induced upregulation of HLA-A2 molecules on TAP-deficient T2 cells.

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  • 1Department of Medical Anatomy, Panum Institute, University of Copenhagen, Denmark.

Abstract

Peptide-induced upregulation of MHC class I on TAP-deficient T2 cells is used to monitor peptide binding to class I molecules. We present a temperature-dependent modification of the T2 assay which increases the sensitivity of peptide binding to MHC class I with several orders of magnitude.

PMID:
8854086
[PubMed - indexed for MEDLINE]
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