Expression of c-fos precedes MDR3 in vincristine and adriamycin selected multidrug resistant murine erythroleukemia cells

Biochem Biophys Res Commun. 1996 Sep 24;226(3):819-21. doi: 10.1006/bbrc.1996.1434.

Abstract

Expression of murine P-glycoprotein (P-gp), encoded by mdrl or mdri3, confers a multidrug resistance phenotype. Higher expression of c-fos and c-jun has also been demonstrated in multidrug resistant human and murine cells. We detected increased expression of c-fos early in the derivation of two series of murine erythroleukemia sublines selected for resistance to vincristine or adriamycin which eventually overexpress mdr3. We speculate that early expression of c-fos prepares cells for overexpression of other genes, such as mdr3, that contribute to the multidrug resistance phenotype.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B*
  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / biosynthesis*
  • ATP-Binding Cassette Transporters / biosynthesis*
  • Animals
  • Blotting, Northern
  • Cell Line
  • Doxorubicin / toxicity*
  • Drug Resistance, Multiple*
  • Gene Expression
  • Genes, fos
  • Genes, jun
  • Humans
  • Leukemia, Erythroblastic, Acute
  • Mice
  • Proto-Oncogene Proteins c-fos / biosynthesis*
  • Proto-Oncogene Proteins c-jun / biosynthesis
  • Tumor Cells, Cultured
  • Vincristine / toxicity*

Substances

  • ATP Binding Cassette Transporter, Subfamily B
  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • ATP-Binding Cassette Transporters
  • Proto-Oncogene Proteins c-fos
  • Proto-Oncogene Proteins c-jun
  • Vincristine
  • Doxorubicin
  • multidrug resistance protein 3