Format

Send to:

Choose Destination
See comment in PubMed Commons below
J Bacteriol. 1996 Oct;178(20):5930-7.

Evolutionary relationships among extradiol dioxygenases.

Author information

  • 1Department of Biochemistry, UniversitĂ© Laval, QuĂ©bec City, Canada. leltis@rsvs.ulaval.ca

Abstract

A structure-validated alignment of 35 extradiol dioxygenase sequences including two-domain and one-domain enzymes was derived. Strictly conserved residues include the metal ion ligands and several catalytically essential active site residues, as well as a number of structurally important residues that are remote from the active site. Phylogenetic analyses based on this alignment indicate that the ancestral extradiol dioxygenase was a one-domain enzyme and that the two-domain enzymes arose from a single genetic duplication event. Subsequent divergence among the two-domain dioxygenases has resulted in several families, two of which are based on substrate preference. In several cases, the two domains of a given enzyme express different phylogenies, suggesting the possibility that such enzymes arose from the recombination of genes encoding different dioxygenases. A phylogeny-based classification system for extradiol dioxygenases is proposed.

PMID:
8830689
[PubMed - indexed for MEDLINE]
PMCID:
PMC178449
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk