Demonstration and definition of the noncovalent binding selectivity of agents related to CC-1065 by an affinity cleavage agent: noncovalent binding coincidental with alkylation

D L Boger; J Zhou; H Cai

doi:10.1016/0968-0896(96)00073-9

Demonstration and definition of the noncovalent binding selectivity of agents related to CC-1065 by an affinity cleavage agent: noncovalent binding coincidental with alkylation

Bioorg Med Chem. 1996 Jun;4(6):859-67. doi: 10.1016/0968-0896(96)00073-9.

Authors

D L Boger¹, J Zhou, H Cai

Affiliation

¹ Department of Chemistry, Scripps Research Institute, La Jolla, CA 92037, USA.

PMID: 8818235
DOI: 10.1016/0968-0896(96)00073-9

Abstract

A study of the DNA cleavage efficiency and selectivity of CDPI3-EDTA (4), an affinity cleavage agent based on the structure of CC-1065, is described. The studies with 4 provide direct evidence of AT-rich noncovalent binding coincidental with all DNA alkylation sites observed with (+)- or ent-(-)-CC-1065.

Publication types

Research Support, U.S. Gov't, P.H.S.

MeSH terms

Alkylation
Antibiotics, Antineoplastic / chemistry
Antibiotics, Antineoplastic / metabolism*
Binding Sites
DNA, Viral / drug effects
DNA, Viral / metabolism
Duocarmycins
Indoles*
Leucomycins / chemistry
Leucomycins / metabolism*
Models, Molecular
Stereoisomerism

Substances

Antibiotics, Antineoplastic
DNA, Viral
Duocarmycins
Indoles
Leucomycins
CC 1065

Grants and funding

CA 41986/CA/NCI NIH HHS/United States