Abstract
A simple antigen detection test was developed for rapid diagnosis of melioidosis caused by Burkholderia (Pseudomonas) pseudomallei infection. The method was based on the use of a specific monoclonal antibody in a sandwich enzyme-linked immunosorbent assay to capture antigen in clinical specimens culture positive for B. pseudomallei. The results showed the sensitivity and specificity of the test to be 75 and 98%, respectively.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal
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Antigens, Bacterial / analysis*
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Bacteriological Techniques / statistics & numerical data
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Burkholderia pseudomallei / immunology*
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Burkholderia pseudomallei / isolation & purification*
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Diagnostic Errors
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Enzyme-Linked Immunosorbent Assay / methods*
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Enzyme-Linked Immunosorbent Assay / statistics & numerical data
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Evaluation Studies as Topic
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Humans
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Melioidosis / diagnosis*
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Sensitivity and Specificity
Substances
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Antibodies, Monoclonal
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Antigens, Bacterial