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Cloning of the human sodium lodide symporter.
Department of Physiology, Ohio State University, Columbus 43210, USA.
The iodide concentrating activity of the thyroid gland is essential to the production of thyroid hormone and also provides a mechanism for the treatment of thyroid cancer by radioiodine ablation. We report here the nucleotide and amino acid sequence of the human sodium iodide symporter (hNIS), which mediates the iodide uptake activity in the thyroid gland. An open reading frame of 1929 nucleotides encodes a protein of 643 amino acids with 84% identity to the rat NIS (rNIS). Transient expression of the hNIS cDNA conferred perchlorate-sensitive iodide uptake to a nonthyroid cell line, COS-7. The expression of hNIS was detected at variable levels in papillary thyroid carcinoma tissues but not in any of the thyroid carcinoma cell lines that have lost the iodide uptake activity.
PMID: 8806637 [PubMed - indexed for MEDLINE]
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Cited by 9 PubMed Central articles
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Expression of the Na+/I- symporter (NIS) is markedly decreased or absent in gastric cancer and intestinal metaplastic mucosa of Barrett esophagus.
Altorjay A, Dohán O, Szilágyi A, Paroder M, Wapnir IL, Carrasco N.
BMC Cancer. 2007 Jan 10; 7:5. Epub 2007 Jan 10.
[BMC Cancer. 2007]
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Cloning and functional identification of slc5a12 as a sodium-coupled low-affinity transporter for monocarboxylates (SMCT2).
Srinivas SR, Gopal E, Zhuang L, Itagaki S, Martin PM, Fei YJ, Ganapathy V, Prasad PD.
Biochem J. 2005 Dec 15; 392(Pt 3):655-64.
[Biochem J. 2005]
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Evidence for transcriptional and posttranscriptional alterations of the sodium/iodide symporter expression in hypofunctioning benign and malignant thyroid tumors.
Trouttet-Masson S, Selmi-Ruby S, Bernier-Valentin F, Porra V, Berger-Dutrieux N, Decaussin M, Peix JL, Perrin A, Bournaud C, Orgiazzi J, et al.
Am J Pathol. 2004 Jul; 165(1):25-34.
[Am J Pathol. 2004]
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