Nat Med. 1996 Sep;2(9):1022-7.

Tethered epidermal growth factor as a paradigm for growth factor-induced stimulation from the solid phase.

Source

Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge 02139, USA.

Erratum in

Nat Med 1997 Jan;3(1):93.

Abstract

We have tethered epidermal growth factor (EGF) to a solid substrate in a manner permitting the factor to retain its biological activity as assessed by both mitogenic and morphological assays. Mouse EGF was covalently coupled to aminosilane-modified glass via star poly(ethylene oxide) (PEO), which allows the ligand to retain significant mobility and active conformation. Tethered EGF was as effective as soluble EGF in eliciting DNA synthesis and cell rounding responses of primary rat hepatocytes under different surface conditions. In contrast, physically adsorbed EGF at comparable surface concentrations showed no activity. Presentation of growth factors in this manner may help to expedite their clinical use by permitting greater control of temporal and spatial availability in the extracellular environment.

PMID:: 8782461; [PubMed - indexed for MEDLINE]

LinkOut - more resources

Supplemental Content

Save items

Related citations in PubMed

Response to transforming growth factor alpha (TGFalpha) and epidermal growth factor (EGF) in hepatocytes: lower EGF receptor affinity of TGFalpha is associated with more sustained activation of p42/p44 mitogen-activated protein kinase and greater efficacy in stimulation of DNA synthesis. [J Cell Physiol. 1998]
Response to transforming growth factor alpha (TGFalpha) and epidermal growth factor (EGF) in hepatocytes: lower EGF receptor affinity of TGFalpha is associated with more sustained activation of p42/p44 mitogen-activated protein kinase and greater efficacy in stimulation of DNA synthesis.
Thoresen GH, Guren TK, Sandnes D, Peak M, Agius L, Christoffersen T. J Cell Physiol. 1998 Apr; 175(1):10-8.
Zonal differences in effects of HGF/SF and EGF on DNA synthesis in hepatocytes under fed or starved conditions. [Am J Physiol. 1998]
Zonal differences in effects of HGF/SF and EGF on DNA synthesis in hepatocytes under fed or starved conditions.
Imai K, Mine T, Tagami M, Hanaoka K, Fujita T. Am J Physiol. 1998 Dec; 275(6 Pt 1):G1394-401.
Effects of exogenous human heparin-binding epidermal growth factor-like growth factor on DNA synthesis of hepatocytes in normal mouse liver. [Biochem Biophys Res Commun. 1999]
Effects of exogenous human heparin-binding epidermal growth factor-like growth factor on DNA synthesis of hepatocytes in normal mouse liver.
Kiso S, Kawata S, Tamura S, Umeki S, Ito N, Tsushima H, Yamada A, Miyagawa J, Higashiyama S, Taniguchi N, et al. Biochem Biophys Res Commun. 1999 Jun 16; 259(3):683-7.
Potentiation of epidermal growth factor-induced DNA synthesis in rat hepatocytes by phenobarbitone: possible involvement of oxidative stress and kinase activation. [Carcinogenesis. 2000]
Potentiation of epidermal growth factor-induced DNA synthesis in rat hepatocytes by phenobarbitone: possible involvement of oxidative stress and kinase activation.
Hodges NJ, Orton TC, Strain AJ, Chipman JK. Carcinogenesis. 2000 Nov; 21(11):2041-7.
Synergistic effects of tethered growth factors and adhesion ligands on DNA synthesis and function of primary hepatocytes cultured on soft synthetic hydrogels. [Biomaterials. 2010]
Synergistic effects of tethered growth factors and adhesion ligands on DNA synthesis and function of primary hepatocytes cultured on soft synthetic hydrogels.
Mehta G, Williams CM, Alvarez L, Lesniewski M, Kamm RD, Griffith LG. Biomaterials. 2010 Jun; 31(17):4657-71. Epub 2010 Mar 21.

See reviews... See all...

Cited by 17 PubMed Central articles

PLLA-PEG-TCH-labeled bioactive molecule nanofibers for tissue engineering. [Int J Nanomedicine. 2011]
PLLA-PEG-TCH-labeled bioactive molecule nanofibers for tissue engineering.
Chen J, Zhou B, Li Q, Ouyang J, Kong J, Zhong W, Xing MM. Int J Nanomedicine. 2011; 6:2533-42. Epub 2011 Oct 21.
Biomaterials that regulate growth factor activity via bioinspired interactions. [Adv Funct Mater. 2011]
Biomaterials that regulate growth factor activity via bioinspired interactions.
Hudalla GA, Murphy WL. Adv Funct Mater. 2011 May 24; 21(10):1754-1768.
Bottom-up signaling from HGF-containing surfaces promotes hepatic differentiation of mesenchymal stem cells. [Biochem Biophys Res Commun. 2011]
Bottom-up signaling from HGF-containing surfaces promotes hepatic differentiation of mesenchymal stem cells.
Ghaedi M, Tuleuova N, Zern MA, Wu J, Revzin A. Biochem Biophys Res Commun. 2011 Apr 8; 407(2):295-300. Epub 2011 Mar 5.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.
Cited in Books
NCBI Bookshelf books that cite the current articles.

Recent activity

Clear Turn Off Turn On

Tethered epidermal growth factor as a paradigm for growth factor-induced stimula...
Tethered epidermal growth factor as a paradigm for growth factor-induced stimulation from the solid phase.
Nat Med. 1996 Sep ;2(9):1022-7.

PubMed
Cue-dependent deficits in grating orientation discrimination after V4 lesions in...
Cue-dependent deficits in grating orientation discrimination after V4 lesions in macaques.
Vis Neurosci. 1996 May-Jun ;13(3):529-38.

PubMed
The prodromal phase of first-episode psychosis: past and current conceptualizati...
The prodromal phase of first-episode psychosis: past and current conceptualizations.
Schizophr Bull. 1996 ;22(2):353-70.

PubMed
Cytokines and adhesion molecules in stroke and related diseases.
Cytokines and adhesion molecules in stroke and related diseases.
J Neurol Sci. 1996 May ;137(2):69-78.

PubMed
Ophthalmia neonatorum associated with maternal chlamydial infections.
Ophthalmia neonatorum associated with maternal chlamydial infections.
Trans Sect Ophthalmol Am Acad Ophthalmol Otolaryngol. 1977 Mar-Apr ;83(2):302-8.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Tethered epidermal growth factor as a paradigm for growth factor-induced stimulation from the solid phase.

Source

Erratum in

Abstract

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Grant Support

LinkOut - more resources

Full Text Sources

Other Literature Sources

Molecular Biology Databases

Supplemental Content

Save items

Related citations in PubMed

Cited by 17 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information