Influence of the 20-kDa protein from Bacillus thuringiensis ssp. israelensis on the rate of production of truncated Cry1C proteins

C Rang; M Bes; V Lullien-Pellerin; D Wu; B A Federici; R Frutos

doi:10.1111/j.1574-6968.1996.tb08395.x

Influence of the 20-kDa protein from Bacillus thuringiensis ssp. israelensis on the rate of production of truncated Cry1C proteins

FEMS Microbiol Lett. 1996 Aug 1;141(2-3):261-4. doi: 10.1111/j.1574-6968.1996.tb08395.x.

Authors

C Rang¹, M Bes, V Lullien-Pellerin, D Wu, B A Federici, R Frutos

Affiliation

¹ BIOTROP-IGEPAM, CIRAD, Montpellier, France.

PMID: 8768532
DOI: 10.1111/j.1574-6968.1996.tb08395.x

Abstract

The potential role of a molecular chaperone on the rate of production of extensively altered Bacillus thuringiensis Cry1C proteins was investigated. Analysis of the proteins produced by the recombinant B. thuringiensis strains showed that the truncated proteins were produced at a low rate. Expression of the 20-kDa protein gene from B. thuringiensis ssp. israelensis in tandem with the truncated-cry1C genes led to the production of a greater amount of proteins. The formation of inclusion bodies, however, did not occur even when the 20-kDa protein gene was expressed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus thuringiensis / genetics*
Bacillus thuringiensis / metabolism*
Bacillus thuringiensis Toxins
Bacterial Proteins / biosynthesis*
Bacterial Proteins / genetics*
Bacterial Toxins*
Cloning, Molecular
Endotoxins / biosynthesis*
Endotoxins / genetics*
Gene Expression Regulation, Bacterial
Hemolysin Proteins
Molecular Chaperones / physiology*
Plasmids
Polymerase Chain Reaction
Recombinant Proteins / biosynthesis
Recombination, Genetic
Restriction Mapping

Substances

Bacillus thuringiensis Toxins
Bacterial Proteins
Bacterial Toxins
Endotoxins
Hemolysin Proteins
Molecular Chaperones
Recombinant Proteins
insecticidal crystal protein, Bacillus Thuringiensis