CTF5--a new transcriptional activator of the NFI/CTF family

Nucleic Acids Res. 1996 Jun 15;24(12):2416-21. doi: 10.1093/nar/24.12.2416.

Abstract

NFI/CTF is a family of polypeptides involved in stimulating the initiation of adenovirus DNA replication and the activation of transcription driven by RNA polymerase II. Several naturally occurring NFI/CTF variants display distinctive transactivation activities in vivo. To define more precisely the role of the NFI/CTF family in regulating gene expression, we cloned the splice variant CTF5, analyzed transcriptional activation patterns in a yeast transcription assay, and compared it with other CTF proteins. CTF5, which lacks exons 9 and 10 including a CTD-like motif essential for transcriptional activation by full-length CTF1, enhances transcription to a greater extent than CTF1. In addition, CTF5 is even more active than CTF7, which lacks exons 7-9. These findings indicate that CTF proteins formed by differential splicing display a much broader range of transcriptional activities as observed previously.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing
  • Amino Acid Sequence
  • Base Sequence
  • CCAAT-Enhancer-Binding Proteins*
  • DNA, Complementary
  • DNA-Binding Proteins / genetics
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • HeLa Cells
  • Humans
  • Molecular Sequence Data
  • NFI Transcription Factors
  • Saccharomyces cerevisiae / genetics
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcriptional Activation

Substances

  • CCAAT-Enhancer-Binding Proteins
  • CTF-1 transcription factor
  • DNA, Complementary
  • DNA-Binding Proteins
  • Fungal Proteins
  • NFI Transcription Factors
  • Transcription Factors

Associated data

  • GENBANK/X92857