Abstract
Vaccination of two chimpanzees against hepatitis B virus (HBV) by intramuscular injection of plasmid DNA encoding the major and middle HBV envelope proteins induced group-, subtype- and preS2-specific antibodies. These were initially of IgM isotype, and then they were of IgG (predominantly IgGl) isotype. The chimpanzee injected with 2 mg of DNA attained >100 milli-international units/ml of anti-HBs antibody after one injection and 14,000 milli-international units/ml after four injections. A smaller dose (400 microg) induced lower and transient titers, but a strong anamnestic response occurred 1 year later. Comparison with responses in 23 chimpanzees receiving various antigen-based HBV vaccines suggests that the DNA approach is promising for prophylactic immunization against HBV.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibody Formation
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DNA, Viral*
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Hepatitis B / immunology*
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Hepatitis B / prevention & control*
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Hepatitis B Antibodies / biosynthesis
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Hepatitis B Antibodies / blood*
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Hepatitis B virus / immunology*
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Immunoglobulin G / biosynthesis
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Immunoglobulin G / blood*
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Immunoglobulin Isotypes / biosynthesis
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Immunoglobulin Isotypes / blood
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Immunoglobulin M / biosynthesis
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Immunoglobulin M / blood*
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Pan troglodytes
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Plasmids
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Time Factors
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Vaccines, Synthetic*
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Viral Envelope Proteins / immunology*
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Viral Vaccines*
Substances
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DNA, Viral
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Hepatitis B Antibodies
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Immunoglobulin G
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Immunoglobulin Isotypes
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Immunoglobulin M
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Vaccines, Synthetic
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Viral Envelope Proteins
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Viral Vaccines