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Proc Natl Acad Sci U S A. 1996 Jul 9;93(14):7063-8.

Bcl-2 interacting protein, BAG-1, binds to and activates the kinase Raf-1.

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  • 1The Burnham Institute, La Jolla, CA 92037, USA.

Abstract

The Bcl-2 protein blocks programmed cell death (apoptosis) through an unknown mechanism. Previously we identified a Bcl-2 interacting protein BAG-1 that enhances the anti-apoptotic effects of Bcl-2. Like BAG-1, the serine/threonine protein kinase Raf-1 also can functionally cooperate with Bcl-2 in suppressing apoptosis. Here we show that Raf-1 and BAG-1 specifically interact in vitro and in yeast two-hybrid assays. Raf-1 and BAG-1 can also be coimmunoprecipitated from mammalian cells and from insect cells infected with recombinant baculoviruses encoding these proteins. Furthermore, bacterially-produced BAG-1 protein can increase the kinase activity of Raf-1 in vitro. BAG-1 also activates this mammalian kinase in yeast. These observations suggest that the Bcl-2 binding protein BAG-1 joins Ras and 14-3-3 proteins as potential activators of the kinase Raf-1.

PMID:
8692945
[PubMed - indexed for MEDLINE]
PMCID:
PMC38936
Free PMC Article
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