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An Mll-AF9 fusion gene made by homologous recombination causes acute leukemia in chimeric mice: a method to create fusion oncogenes.
Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom.
Homologous recombination in embryonal stem cells has been used to produce a fusion oncogene, thereby mimicking chromosomal translocations that frequently result in formation of tumor-specific fusion oncogenes in human malignancies. AF9 sequences were fused into the mouse Mll gene so that expression of the Mll-AF9 fusion gene occurred from endogenous Mll transcription control elements, as in t(9;11) found in human leukemias. Chimeric mice carrying the fusion gene developed tumors, which were restricted to acute myeloid leukemias despite the widespread activity of the Mll promoter. Onset of perceptible disease was preceded by expansion of ES cell derivatives in peripheral blood. This novel use of homologous recombination formally proves that chromosomal translocations contribute to malignancy and provides a general strategy to create fusion oncogenes for studying their role in tumorigenesis.
PMID: 8681380 [PubMed - indexed for MEDLINE]
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Cited by 44 PubMed Central articles
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Microenvironment determines lineage fate in a human model of MLL-AF9 leukemia.
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[Cancer Cell. 2008]
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Robinson BW, Cheung NK, Kolaris CP, Jhanwar SC, Choi JK, Osheroff N, Felix CA.
Blood. 2008 Apr 1; 111(7):3802-12. Epub 2008 Jan 14.
[Blood. 2008]
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