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EMBO J. 1996 Jul 1;15(13):3332-7.

A splice variant of the neurotrophin receptor trkB with increased specificity for brain-derived neurotrophic factor.

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  • 1Max-Planck-Institute for Psychiatry, Department of Neurobiochemistry, Planegg-Martinsreid, Germany.

Abstract

The trkB gene codes for a receptor tyrosine kinase, which is essential for the development of the peripheral nervous system. This receptor can be activated by three different neurotrophins: BDNF, NT-4/5 and NT-3. The extracellular domain of trkB was found to be encoded in 10 exons corresponding to receptor subdomains previously identified on the basis of protein sequence comparisons. Exon 9 was skipped in a novel tyrosine kinase transcript of the trkB gene, designated ctrkB-Short (ctrkB-S). While the previously described trkB receptor ctrkB-Long (ctrkB-L) and trkB-S receptors were activated similarly by BDNF, trkB-S interacted poorly with NT-4/5 and NT-3 as measured by ligand binding, ligand-induced autophosphorylation and ligand-dependent activation of p21ras. Efficient activation of ctrkB-S by NT-3 was restored by a single amino acid replacement in NT-3 (D15A). Both trkB-L and trkB-S transcripts were detected in embryonic neurons.

PMID:
8670834
[PubMed - indexed for MEDLINE]
PMCID:
PMC451896
Free PMC Article
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