Send to

Choose Destination
See comment in PubMed Commons below
J Urol. 1996 Jul;156(1):261-6.

TGF-beta mRNA expression in the renal pelvis after experimental and clinical ureteropelvic junction obstruction.

Author information

  • 1Division of Urology, Children's Memorial Hospital, Chicago, Illinois, USA.



The renal parenchymal expression of transforming growth factor-beta (TGF-beta) is increased in experimental obstructive uropathy. To better understand the pathophysiology of hydronephrosis, we pursued this observation by determining if the expression of TGF-beta is also increased in the obstructed renal pelvis.


The expression of TGF-beta mRNA was evaluated in experimental and clinical ureteropelvic junction obstruction by reverse transcriptase polymerase chain reaction (RT-PCR). Southern blot analysis with labeled human cDNA probes for TGF-beta was then done to verify the results.


Experimentally, pelves subjected to ureteral constriction showed significantly higher TGF-beta mRNA expression than normals. After reversal of obstruction, the expression of TGF-beta remained significantly higher than normals but was not different from pelves with ongoing obstruction. Clinically, there was a significantly higher level of TGF-beta mRNA expression in obstructed pelves than in nonobstructed ureters. High levels of TGF-beta mRNA expression correlated significantly with good clinical outcome, good renal function, muscle hypertrophy and acute onset of obstruction.


There is increased TGF-beta mRNA expression in the renal pelvis following clinical and experimental ureteropelvic junction obstruction. These data implicate TGF-beta in the adaptive molecular responses that increase muscle and collagen elaboration seen in hydronephrosis.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk