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Biochem Biophys Res Commun. 1996 Mar 18;220(2):280-4.

A new plasmid-encoded proteic killer gene system: cloning, sequencing, and analyzing hig locus of plasmid Rts1.

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  • 1Department of Bacteriology, Shinshu University School of Medicine, Japan.

Abstract

A new proteic killer gene system, hig, was identified on the plasmid Rts1. The hig locus consisting of a higA and higB is directly related to the temperature sensitive host cell growth conferred by Rts1. We proved that higB encoding presumably a 92-amino-acid polypeptide inhibited segregation of plasmid free cells, and higA encoding a 104-amino-acid polypeptide suppressed the higB function both in cis and in trans.

PMID:
8645296
[PubMed - indexed for MEDLINE]
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